Instruction Manual_Rev.Cに記載が間違っているところがあります。
次のように訂正します。
p.4
Solution 3 3.1 Solution
3. First-dimension sample buffer
(誤) 8.0M Urea 5.7g
1.6% Bio-Lyte 5/7 ampholyte 400 ul
0.4% Bio-Lyte 3/10 ampholyte 100 ul
(正) 8.0M Urea 4.8g
1.6% Bio-Lyte 5/7 ampholyte 400 ul(40% Bio-Lyte5/7)
0.4% Bio-Lyte 3/10 ampholyte 100 ul(40% Bio-Lyte3/10)
4.First-dimension sample overlay buffer
(誤) 4.0M Urea 5.41g
0.8% Bio-Lyte 5/7 ampholyte 200 ul
0.2% Bio-Lyte 3/10 ampholyte 50 ul
(正) 4.0M Urea 2.4g
0.8% Bio-Lyte 5/7 ampholyte 200 ul(40% Bio-Lyte5/7)
0.2% Bio-Lyte 3/10 ampholyte 50 ul(40% Bio-Lyte3/10)
p.5
Solution 3 3.2 Preparation of First- Dimension Tube Gels
1. First-dimension gel monomer solution
(誤) 8.0M Urea 5.5g
(正) 8.0M Urea 4.8g