Helios Gene Gunシステムに関する質問集

質問 ID: 1081

[Helios Gene Gunシステム] 培養細胞に導入できますか?

回答

導入できます。
Dishなどに入れて遺伝子導入を行い、導入可能面積は2平方cm程度です。

以下のような導入文献がありますのでご参照ください。


In vivo and in vitro effects of two novel gamma-actin (ACTG1) mutations that cause
DFNA20/26 hearing impairment
Matías Morín, Keith E. Bryan, Fernando Mayo-Merino, Richard Goodyear, Ángeles Mencía, Silvia Modamio-Høybjør, Ignacio del Castillo, Jessica M. Cabalka, Guy
Richardson, Felipe Moreno, Peter A. Rubenstein, and Miguel Ángel Moreno-Pelayo
Hum. Mol. Genet., Aug 2009; 18: 3075 - 3089.

Twinfilin 2 Regulates Actin Filament Lengths in Cochlear Stereocilia
Anthony W. Peng, Inna A. Belyantseva, Patrick D. Hsu, Thomas B. Friedman, and
Stefan Heller
J. Neurosci., Dec 2009; 29: 15083 - 15088.

D -Catenin Signaling Pathway Leading to Dendritic Protrusions
Kawther Abu-Elneel, Tomoyo Ochiishi, Miguel Medina, Monica Remedi, Laura
Gastaldi, Alfredo Caceres, and Kenneth S. Kosik
J. Biol. Chem., Nov 2008; 283: 32781 - 32791.

Aminoglycoside-Induced Phosphatidylserine Externalization in Sensory Hair Cells
Is Regionally Restricted, Rapid, and Reversible
Richard J. Goodyear, Jonathan E. Gale, Kishani M. Ranatunga, Corné J. Kros, and
Guy P. Richardson
J. Neurosci., Oct 2008; 28: 9939 - 9952.

Developmental Regulation of Sensory Neurite Growth by the Tumor Necrosis Factor
Superfamily Member LIGHT
Núria Gavaldà, Humberto Gutierrez, and Alun M. Davies
J. Neurosci., Feb 2009; 29: 1599 - 1607.