質問 ID：2949
[Helios Gene Gunシステム] 蛍光色素を導入した例はありますか？
回答
神経細胞を蛍光色素ラベルした文献が多数あります。
一例として、下記文献をご参照ください。
Multicolor "DiOlistic"; Labeling of the Nervous System Using Lipophilic Dye Combinations
Wen-Biao Gan, Jaime Grutzendler, Wai Thong Wong, Rachel O. Wong, and Jeff W. Lichtman
Neuron, Vol. 27, 219-225, August, 2000
Reversal of long-term dendritic spine alterations in Alzheimer disease models
Donna L. Smith, Julio Pozueta, Bing Gong, Ottavio Arancio, and Michael Shelanski
PNAS, Sep 2009; 106: 16877 - 16882.
Loss of GluN2B-Containing NMDA Receptors in CA1 Hippocampus and Cortex Impairs Long-Term Depression, Reduces Dendritic Spine Density, and Disrupts Learning
Jonathan L. Brigman, Tara Wright, Giuseppe Talani, Shweta Prasad-Mulcare, Seiichiro Jinde, Gail K. Seabold, Poonam Mathur, Margaret I. Davis, Roland Bock, Richard M. Gustin, Roger J. Colbran, Veronica A. Alvarez, Kazu Nakazawa, Eric Delpire, David M. Lovinger, and Andrew Holmes
J. Neurosci., Mar 2010; 30: 4590 - 4600.
Ballistic Delivery of Dyes for Structural and Functional Studies of the Nervous System
Wen-Biao Gan, Jaime Grutzendler, Rachel O. Wong, and Jeff W. Lichtman
Cold Spring Harb Protoc, Apr 2009; 2009: pdb.prot5202.
一例として、下記文献をご参照ください。
Multicolor "DiOlistic"; Labeling of the Nervous System Using Lipophilic Dye Combinations
Wen-Biao Gan, Jaime Grutzendler, Wai Thong Wong, Rachel O. Wong, and Jeff W. Lichtman
Neuron, Vol. 27, 219-225, August, 2000
Reversal of long-term dendritic spine alterations in Alzheimer disease models
Donna L. Smith, Julio Pozueta, Bing Gong, Ottavio Arancio, and Michael Shelanski
PNAS, Sep 2009; 106: 16877 - 16882.
Loss of GluN2B-Containing NMDA Receptors in CA1 Hippocampus and Cortex Impairs Long-Term Depression, Reduces Dendritic Spine Density, and Disrupts Learning
Jonathan L. Brigman, Tara Wright, Giuseppe Talani, Shweta Prasad-Mulcare, Seiichiro Jinde, Gail K. Seabold, Poonam Mathur, Margaret I. Davis, Roland Bock, Richard M. Gustin, Roger J. Colbran, Veronica A. Alvarez, Kazu Nakazawa, Eric Delpire, David M. Lovinger, and Andrew Holmes
J. Neurosci., Mar 2010; 30: 4590 - 4600.
Ballistic Delivery of Dyes for Structural and Functional Studies of the Nervous System
Wen-Biao Gan, Jaime Grutzendler, Rachel O. Wong, and Jeff W. Lichtman
Cold Spring Harb Protoc, Apr 2009; 2009: pdb.prot5202.